Technology of Molecular Diagnosis

Technology Details

Sample Processing Solution Technology specialized for Field Diagnostics

1. Non-Powered Microfluid-based
Nucleic acid Extraction Technology

Microfluidic Paper Chip based Extracting
Nucleic Acid

Using paper microfluidic chips, patient’s sample
(virus, etc)
Through the process of Lysis, Capturing,
Hibrid DNA: RNA, extract nulecic acid

2. Simultaneous Multi-diagnosis of Isothermal Amplification

Simultaneous Multiple Target Primer Technology
Simultaneous multiple target primer technology for isothermal amplification is appropriate for Various reaction condition based on each target gene Able to fusion with various diagnosis device with minimizing amplification error and optimal specification

3. iNAD Diagnostic Technology

More rapidly and sensitively than real-time PCR
By combining RPA isothermal amplification with iNAD using bio-optical sensors, Severe Fever with Thrombocytopenia Syndrome (SFTS) and Tsutsugamushi infection target The results of clinical validity assessments,
Faster and higher sensitivity than RT-PCR
(Mini-POCT Device Possible)

4. Amplification technology
on paper chips

Paper-Microfluidic Chip based INAA Technology
- Low-cost high-speed high-sensitivity At home
diagnosis paper chip technology
Using paper microfluid chips, isothermal
amplification technology
Quickly and accurately diagnose causative
bacteria at home
- Optimized for isothermal acid amplification
Establish various specific condition such as paper
chip structure, fixation of chemical components,
PH, buffer components, and temperature

5. Paper based all-in-one molecular diagnostics

All in One based Molecular Diagnostic Structure
Technology

Lab-on-paper for all-in-one molecular
diagnostics of zika, dengue,
and chikungunya virus from human serum

6. Commercial Application

Fabrication Technology
that implements preparation and amplification
in paper chips

Platform Technology

Understanding and Application of LAB on Paper

Compare Product/Commercialization

Comparison of Competitive Products and Schedule of Commercialization

1. Comparison with other products

Division Seegene Product AN Product S Product
RT-PCR NINA Rapid Antigen
1 Analysis method Molecular Diagnosis Molecular Diagnosis(Paper Biochip) Immune Diagnosis
2 Reaction Temperature Thermocycling Single Temperature Room Temperature
3 Analysis Time 2~3 Hours Less than 30 mins 15~20 mins
4 Minimum Detection Limit 1.0~10 copies 100 copies -
5 Equipment Real time PCR No No
6 Sensitivity 98% 98% 50%
7 Specificity 98% 98% 35%
8 At Home diagnosis Impossible Possible Possible

2. Comparison of competing product

MEMS Technology is easy to commercialize but expensive.
Paperchip Technology needs high-end technologies but get best competition.

Division AccuFast™ L Company COVID-19
1 Analysis method Molecular Diagnostic based on Paper Biochip Molecular Diagnostic based on MEMS
2 Reaction Temperature Single Temperature (60℃~) Single Temperature (60℃~)
3 Analysis Time Less than 30 mins Less than 30 mins
4 Minimum
Detection Limit
100 copies/reaction 100 copies/reaction
5 Sensitivity 98~100%* 97%**
6 Specificity 98~100%* 98%**
7 Visual Detection Colorimetric Fluorescence
8 At home diagnosis Yes Yes
9 Supply Price 13 USD / test 50 USD / test

*AN intra data **L사 homepage.